Peptidoglycan hydrolases, a family of enzymes involve in peptidoglycan biosynthesis and metabolism must be tightly regulated since they are capable of causing lysis; hence regulation must not limit their role in the enlargement and modification of the bacterial cell wall during cell growth and division. Regulation was controlled at the transcriptional level via control of enzyme activity and also affected by the physico-chemical properties of the peptidoglycan hydrolase’s environment. In this study, a SA1825::lacZ reporter gene fusion was created in the S. aureus SH1000 background as well as the transfer of sagA::lacZ reporter gene fusion from S. aureus RN4220 into SH1000. The expression of four lacZ reporter gene fusions of putative N-acetylglucosaminidase encoding genes known as atl, sagA, scaH and SA1825 in S. aureus SH1000 background was comparatively studied in media with and without 1M NaCl. All four S. aureus glucosaminidase genes were found to be maximally expressed during the exponential phase of growth with atl showed the highest expression in the absence of 1M NaCl. However in the presence of 1M NaCl, the expression of the four genes was showed to be higher, the kinetics of gene expression remained generally comparable but the overall growth rate was reduced. This upregulation pattern in N-acetylglucosaminidase genes expression suggests the necessity for enhanced peptidoglycan activity that will facilitate alteration of cell wall in response to higher osmotic environment.