Staphylococal infection spread in the hospital especially as a nosocomial infection could be controlled by the using of proper antiseptic by the medical staff. This study carried out impacts of contact time and concentration chlorhexidine to methicillin staphylococcus aureus (mrsa) growth use in various contact time and concentration. chlorhexidine 0.25%, 0.5%, 1%, 2% and 4% had been tested to mrsa isolate in various contact time i.e. 30", 60", 90", 120". control group were mrsa contacted each to 0% chlorhexidine. All treatment replicated 4 times. chlorhexidine inhibiton effect compared to control group. the result showed there were different inhibition effects by chlorhexidine to mrsa growth in the control group of all concentrations. significance different in contact time only happened between 30", 60" and 90". The conclusion of the investigation shpwed that 0.25% concentration of chlorhexidine and 90" contact time were the most effective concentration and contact time for inhibition methicillin resistant staphylococcus aureus growth.
Key word: chlorhexidine, concentration, contact time, mrsa